NASC

NASC ID: N849682

Name: WiscDsLox247B12

ABRC stock number: CS849682

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox247B12.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G22788 (chr 5 pos 7603545 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796606.

Wiscseq_DsLox247B12.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G77330.1-P355 and AT1G77340.1-UTR3 (at chr 1 pos 29066130 (C/29066130-29066213) on the TAIR10). The sequences' 1-84 bps mapped on the genome, 84-100 bps onto the T-DNA. Pools P 0, R 6, C 6 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452084.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox247B12

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G77330 AT1G77340 AT5G22788